Abstract
Contamination of soil by petroleum hydrocarbon compounds could
affect microbial communities due to its toxicity and impact to soil fertility.
The purpose of this research is to study microbial communities in soil
contaminated by petroleum hydrocarbon compounds. This research was conducted by
taking crude-contaminated soil sample from the areas planted with Acacia sp.,
H. brasiliensis, and S. mahagoni. Microbes were isolated from the soil and
purified by using SMSS media added with the used-oil with concentration of 2%
and 4% to study the effect of the used-oil to microbial communities. The
results of microscopical identification show that in Acacia sp. soil was
found 1 species (A. sclerotiorum), H. brasiliensis found 2
species (A. niger dan Penicillium sp.), and S. mahagoni found
3 species (A. niger, Penicillium sp. dan A. fumigatus). All of the isolates can use
the used-oil at concentration of 2% as carbon source. At concentration of 4%,
only A. niger could grow well. According to the results petroleum hydrocarbon
compounds affect microbial communities but the communities were not affected by
the kind of plants grown on in the contaminated soil.
Keywords: microbial community, petroleum hydrocarbon compound, A. sclerotiorum, A. niger,
Penicillium sp., A. fumigatus
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